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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Thromboxane A2
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Thromboxane A2, TX-A2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Thromboxane A2,TX-A2,
Search name
Rat Thromboxane A2 ELISA Kit,Rat TX-A2 ELISA Kit,Thromboxane A2 ELISA Kit,TX-A2 ELISA Kit,ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of thromboxane a2,Thromboxane A2 concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Thromboxane A2, During the reaction, Thromboxane A2 in the sample or standard competes with
a fixed amount of biotin-labeled Thromboxane A2 for sites on a pre-coated Monoclonal antibody specific to Thromboxane A2. Excess
conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is
added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Thromboxane A2 in the samples is then determined by comparing the O.D. of the samples to the
standard curve.
PDF manual download
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文献和实验Assessment of Cellular Localization of the Thromboxane A2 Receptor by Immunocytochemistry
Transmembrane receptors play a crucial role in communication between cells. By coupling to intracellular proteins and second-messenger systems, these molecular entities allow for the transduction of a biochemical message across the cell
本文主要介绍 ELISA 试剂盒、实验样本、相关问题,帮助大家更好地开展实验 试剂盒 Q1:科研试剂盒与临床试剂盒的区别 ? A1:临床试剂是经过权威机构认可的,如 FDA、CFDA 等;而科研试剂一般是厂家自主研制,不需要通过权威机构验证。 经营临床诊断类产品的机构需要取得《医疗器械经营企业许可证》;经营科研试剂的机构不需要此证件。 临床试剂盒一般仅用于检测人血清/血浆等较为单一的分泌性样本类型;科研试剂盒的样本检测种类、检测范围则比临床试剂盒宽广得多。但一般来说,临床试剂盒的质量
大鼠腺苷 A2b 受体 (A2b R)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 A2b R 单抗包被于酶标板上,标准品和样品中的 A2b R 与单抗结合,加入生物素化的抗大鼠 A2b R ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸
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