- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0922r
- 2025年11月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Vitamin E
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Vitamin E ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Vitamin E,VE
Search name
Rat Vitamin E ELISA Kit, Rat VE ELISA Kit, Vitamin E ELISA Kit, VE ELISA Kit, Rat Nutrition Tocopherol ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Vitamin E concentrations in serum, plasma, tissue homogenates,
cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Vitamin E, During the reaction, Vitamin E in the sample or standard competes with a fixed amount
of biotin-labeled Vitamin E for sites on a pre-coated Monoclonal antibody specific to Vitamin E. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Vitamin E in the samples is then determined by comparing the O.D. of the samples to the standard curve
PDF manual download
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文献和实验缺乏维生素 E，就会阻碍受精卵在子宫内着床后的发育，可使精细胞变性，发生退化，引起不孕症。此外，还能引起肌肉萎缩和运动麻痹。在许多哺乳动物中都可见到 E缺乏症，但在人体中，还没有得到确实的 E缺乏症的证据，一般在生物体内有防止维生素 A、胡萝卜素、脂肪等氧化的作用。亦有报道其磷酸脂可抑制琥珀酸氧化系统和抑制磷酸酶的作用，但都还未最后确定。成人每天需要量约为 30毫克的混合生育酚。定量法有吸收测定和比色法等，但因而为α，β，γ、δ都作为同一效力被测定，所以测定数值比生物学效力高，所以并不是太好的方法。
Supercritical Fluid Aerosolized Vitamin E Supplementation
Rapid release of the applied pressure on a supercritical fluid solution allows the fluid to expand, its solvent strength to drop, and solute nucleation to occur, forming fine, airborne particles. This phenomenon was first observed
Tocopherols (vitamin E) are the major lipid-soluble chain-breaking antioxidants of membranes and blood plasma ( 1 – 3 ). Antioxidant function of tocopherols is confined to their chromanol nucleus with 6-hydroxy group, and is based
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