- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0876r
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Calcitonin gene-related peptide,CGRP
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Calcitonin gene- related peptide, CGRP ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Calcitonin gene-related peptide 1,; Alpha-type CGRP,Calcitonin gene-related peptide I(CGRP-I),; Calcitonin,alpha-type CGRP; CALC1;
CGRP1; Calcitonin 1; calcitonin/calcitonin-related polypeptide, alpha; katacalcin; calcitonin-related polypeptide alpha
Search name
Rat Calcitonin gene-related peptide 1 ELISA Kit, Rat CGRP ELISA Kit, Rat Calcitonin gene-related peptide ELISA Kit, Rat
Alpha-type CGRP ELISA Kit, Rat Calcitonin gene-related peptide I ELISA Kit, Rat CGRP-I ELISA Kit, Rat Calcitonin,alpha-type
CGRP ELISA Kit, Rat CALC1 ELISA Kit, Rat CGRP1 ELISA Kit, Rat Calcitonin 1 ELISA Kit, Rat calcitonin ELISA Kit, Rat katacalcin
ELISA Kit, Rat calcitonin-related polypeptide alpha ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat calcitonin gene-related peptide 1,CGRP concentrations in
serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CGRP. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CGRP and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain CGRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CGRP in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Characterization of CGRP Receptor Binding
for calcitonin gene‐related peptide (CGRP) and islet amyloid polypeptide (IAPP) in mammalian lung: Species variation and binding of truncated CGRP and IAPP. Endocrinology. 133:2351‐2361.
Production of Antisera Using Peptide Conjugates
of different crosslinking agents utilized for the coupling of peptides to carrier proteins; examples of each type are covered in this chapter. In the case of larger neuropeptides, such as calcitonin gene-related peptide (CGRP
in four different osteoblastic cell lines and in isolated neonatal mouse calvarial bone cells has been examined. In the rat osteosarcoma cell line UMR-106-01, vasoactive intestinal polypeptide (VIP, 0.001-1 microM), calcitonin gene-related peptide (CGRP, 0.3-30 nM
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