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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat thrombin-antithrombin complex,TAT
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat thrombin- antithrombin complex, TAT ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TATC,Thrombin-antithrombin complex, Thrombin-antithrombin,TAT, Thrombin, antithrombin
Search name
Rat TATC ELISA KIT ,Rat Thrombin-antithrombin complex ELISA KIT ,Rat Thrombin-antithrombin ELISA KIT ,Rat TAT ELISA KIT ,Rat Thrombin ELISA KIT ,Rat antithrombin ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Thrombin-antithrombin complex concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombin-antithrombin complex. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Thrombin-antithrombin complex and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Thrombin-antithrombin complex, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Thrombin-antithrombin complex in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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