- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0656r
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat diamine oxidase,DAO
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat diamine oxidase, DAO ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Amiloride-sensitive amine oxidase [copper-containing],DAO,Diamine oxidase,Kidney amine oxidase,KAO,Amiloride-binding protein,ABP,Histaminase, DAO,AOC1,ABP; ABP1; DAO1; KAO; amiloride binding protein 1 amine oxidase (copper-containing); amiloride-binding protein 1; amiloride-sensitive amine oxidase; amiloride-sensitive amine oxidase [copper-containing]; amine oxidase copper domain-containing protein 1; diamine oxidase; histaminase; kidney amine oxidase; amine oxidase copper containing 1
Search name
Rat Amiloride-sensitive amine oxidase [copper-containing] ELISA KIT ,Rat DAO ELISA KIT ,Rat Diamine oxidase ELISA KIT ,Rat Kidney amine oxidase ELISA KIT ,Rat KAO ELISA KIT ,Rat Amiloride-binding protein ELISA KIT ,Rat ABP ELISA KIT ,Rat Histaminase ELISA KIT ,Rat DAO ELISA KIT ,Rat AOC1 ELISA KIT ,Rat ABP ELISA KIT ,Rat ABP1 ELISA KIT ,Rat DAO1 ELISA KIT ,Rat KAO ELISA KIT ,Rat amiloride binding protein 1 amine oxidase (copper-containing) ELISA KIT ,Rat amiloride-binding protein 1 ELISA KIT ,Rat amiloride-sensitive amine oxidase ELISA KIT ,Rat amiloride-sensitive amine oxidase [copper-containing] ELISA KIT ,Rat amine oxidase copper domain-containing protein 1 ELISA KIT ,Rat diamine oxidase ELISA KIT ,Rat histaminase ELISA KIT ,Rat kidney amine oxidase ELISA KIT ,Rat amine oxidase copper containing 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat amiloride-sensitive amine oxidase [copper-containing],DAO concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DAO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DAO and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DAO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DAO in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
signal. However, this process required more time. Under these conditions, a hybridizing 2-kb band was detected in the kidney and cerebellum of the mouse, and in the kidney, liver, and hindbrain of the rat. 10 , 11 ). D-Amino-acid oxidase protein
Methods for the Detection of D-Amino-Acid Oxidase
, and in the kidney, liver, and hindbrain of the rat. 10 , 11 ). D-Amino-acid oxidase protein was detected by western blotting in the kidney and brain of the mouse, and in the kidney of the rat ( 12 - 14 ). RT-PCR amplified a D-amino-acid oxidase cDNA
Histamine N-Methyltransferase: Enzymology and Functional Aspects
, HNMT has not been the subject of a comprehensive review or analysis. This is surprising since HNMT and diamine oxidase (DAO; EC 1.3.4.6) are the only known enzymes that terminate the physiologic activity of histamine (HA) in mammals. However, to a large
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