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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Super Oxidase Dimutase
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Superoxide dismutase, SOD ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Superoxide dismutase [Cu-Zn], ALS,ALS1,IPOA,SOD,hSod1,homodimer,Cu/Zn superoxide dismutase,SOD, soluble,indophenoloxidase
A,superoxide dismutase [Cu-Zn],superoxide dismutase, cystolic,superoxide dismutase 1 soluble,SOD1
Search name
Rat SOD ELISA Kit,Rat SOD1 ELISA Kit, Rat Superoxide dismutase ELISA Kit,ELISA Kit,SOD1 ELISA Kit, Superoxide dismutase
ELISA Kit, Superoxide dismutase [Cu-Zn] ELISA kit, ALS ELISA kit,ALS1 ELISA kit,IPOA ELISA kit,SOD ELISA kit,Sod1 ELISA
kit,homodimer ELISA kit,Cu/Zn superoxide dismutase ELISA kit,SOD ELISA kit, soluble ELISA kit,indophenoloxidase A ELISA
kit,superoxide dismutase [Cu-Zn] ELISA kit,superoxide dismutase ELISA kit, cystolic ELISA kit,superoxide dismutase 1 soluble
ELISA kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat superoxide dismutase [cu-zn],Superoxide dismutase [Cu-Zn]
concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide dismutase [Cu-Zn]. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for
Superoxide dismutase [Cu-Zn] and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. Only those wells that contain Superoxide dismutase [Cu-Zn], biotin-conjugated
antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Superoxide dismutase [Cu-Zn] in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
signal. However, this process required more time. Under these conditions, a hybridizing 2-kb band was detected in the kidney and cerebellum of the mouse, and in the kidney, liver, and hindbrain of the rat. 10 , 11 ). D-Amino-acid oxidase protein
Methods for the Detection of D-Amino-Acid Oxidase
, and in the kidney, liver, and hindbrain of the rat. 10 , 11 ). D-Amino-acid oxidase protein was detected by western blotting in the kidney and brain of the mouse, and in the kidney of the rat ( 12 - 14 ). RT-PCR amplified a D-amino-acid oxidase cDNA
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
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