Rat Interleukin- 1, IL- 1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Interleukin-1 beta(IL-1 beta),; IL-1 beta; IL-1; IL1-BETA; IL1F2; catabolin; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta;
interleukin 1, beta
Search name
Rat Interleukin-1 ELISA Kit, Rat IL-1 ELISA Kit, Rat Interleukin-1 beta ELISA Kit, Rat IL-1 beta ELISA Kit, Rat IL1F2 ELISA Kit, Rat
catabolin ELISA Kit, Rat preinterleukin 1 beta ELISA Kit, Rat pro-interleukin-1-beta ELISA Kit, Rat interleukin 1 beta ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat interleukin-1 beta,IL-1 beta concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1 beta. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-1 beta and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain IL-1 beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-1 beta in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
PDF manual download
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