- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0507r
- 2025年11月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Leukotriene E4,LTE4
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Leukotriene E4, LTE4 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Leukotriene E4,LTE4
Search name
Rat Leukotriene E4 ELISA Kit, Rat LTE4 ELISA Kit, Leukotriene E4 ELISA Kit, LTE4 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Leukotriene E4,LTE4 concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to LTE4, During the reaction, LTE4 in the sample or standard competes with a fixed amount of
biotin-labeled LTE4 for sites on a pre-coated Monoclonal antibody specific to LTE4. Excess conjugate and unbound sample or standard
are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTE4 in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人白细胞三烯E4 ( LTE4 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 LTE4 单抗包被于酶标板上，标准品和样品中的 LTE4与单抗结合，加入生物素化的抗人
Extraction of Eicosanoids from Biological Fluids, Cells, and Tissues
as a result of the presence of the 20 carbon chain. Exceptions to this are the cysteinyl-leukotrienes (cys-LTs), which have positively charged amino groups. Thus the partitioning of leukotrienes C4 , D4 , and E4 and their metabolites between these two phases
We have summarized in Chapter 16 the recent advances in the mechanisms of regulation of leukotriene and prostaglandin biosynthesis and their pathways of synthesis. This chapter focuses on the measurement of the leukotrienes and other LOX
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