- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0118r
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Cluster of differentiation 30 ligand,CD30L
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Cluster of differentiation 30 ligand, CD30L ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Tumor necrosis factor ligand superfamily member 8,CD30 ligand(CD30-L), CD30 ligand,CD30L; CD153; CD30LG; CD153 antigen; CD30
antigen ligand; CD30-L; tumor necrosis factor ligand superfamily member 8; tumor necrosis factor (ligand) superfamily, member 8
Search name
Rat Tumor necrosis factor ligand superfamily member 8 ELISA Kit, Rat TNFL8 ELISA Kit, Rat CD30 ligand ELISA Kit,
Rat Cluster of differentiation 30 ligand ELISA Kit, Rat CD30L ELISA Kit, Rat CD30-L ELISA Kit, Rat CD153 ELISA
Kit, Rat CD30LG ELISA Kit, Rat CD153 antigen ELISA Kit, Rat D30 antigen ligand ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat tumor necrosis factor ligand superfamily member
8,TNFL8 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFL8. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TNFL8 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain TNFL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TNFL8 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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文献和实验人OX40 Ligand(OX40L)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人OX40 Ligand ( OX40L )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 OX40L/TNFSF4 单抗包被于酶标板上，标准品和样品中的 OX40L与单抗结合，加入
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
离心 10min（建议两次），快速获得澄清上清。 3. 广泛兼容适配强：完美适配 ELISA、Luminex 等主流检测平台，无需反复优化方案，提升实验灵活性与可重复性。 二、关键环节：蛋白定量实现样本调平 组织样本个体差异大，蛋白浓度参差不齐，未定量直接上样会导致结果失真。裂解后需通过 BCA 法精准定量： 1. 取裂解上清，参考 abs9232 试剂盒说明书配置标准品与反应液； 2. 按步骤孵育后检测吸光度，绘制标准曲线并计算样本蛋白浓度； 3. 将所有样本统一稀释至 1μg/μl 左右，确保
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