Rat Matrix metalloproteinase 1

Rat Matrix metalloproteinase 13, MMP13 ELISA kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MMP13,CLG3; MANDP1; MMP-13; collagenase 3; matrix metallopeptidase 13; matrix metalloproteinase 13; matrix metallopeptidase 13, Matrix metalloproteinase-13, UMRCASE,

Search name

Rat MMP13 ELISA KIT ,Rat CLG3 ELISA KIT ,Rat MANDP1 ELISA KIT ,Rat MMP-13 ELISA KIT ,Rat collagenase 3 ELISA KIT ,Rat matrix metallopeptidase 13 ELISA KIT ,Rat matrix metalloproteinase 13 ELISA KIT ,Rat matrix metallopeptidase 13 ELISA KIT ,Rat Matrix metalloproteinase-13 ELISA KIT ,Rat UMRCASE ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat Collagenase 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagenase 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Collagenase 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Collagenase 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Collagenase 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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