- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- CELI-66106r
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Angiotensin II,ANG2
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Angiotensin II, ANG2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Angiotensin II, ANG II
Search Name
Rat Angiotensin II ELISA Kit, Rat ANG II ELISA Kit,Angiotensin II ELISA Kit,ANG II ELISA Kit, ANG2 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of angiotensin ii,Angiotensin II concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Angiotensin II, During the reaction, Angiotensin II in the sample or standard competes with a fixed
amount of biotin-labeled Angiotensin II for sites on a pre-coated Monoclonal antibody specific to Angiotensin II. Excess conjugate and
unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Angiotensin II in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Characterization of Angiotensin II Receptors
. Chang, R.S.L., Lotti, V.J., Chen, T.B., and Faust, K.A. 1990. Two angiotensin II binding sites in rat brain revealed using 125I‐Sar1‐Ile8‐angiotensin II and selective nonpeptide antagonists. Biochem. Biophys
James Hardwick angiotensin assay protocol
2 mMolar [Val5]-angiotensin II 3. Remove 5 microliter aliquots of the reaction mixture after 1, 3, and 5 minutes of reaction time. The incorporation of ATP is linear during this period. The 5 microliter aliquots
The Detection of Antibodies to the Angiotensin II-Type 1 Receptor in Transplantation
The detection of auto-antibodies detection is a major aspect of patient’s immunomonitoring. Antibodies directed against the heart and kidney Angiotensin II type 1 receptor (AT1 R) play a major role in antibody mediated rejection after heart
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