- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- CELI-66017r
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Estrone,E1
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Estrone, E1 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Estrone,Oestrone, E1
Search name
Rat Estrone ELISA Kit, Rat Oestrone ELISA Kit, Rat E1 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Estrone concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Estrone, During the reaction, Estrone in the sample or standard competes with a fixed amount of
biotin-labeled Estrone for sites on a pre-coated Monoclonal antibody specific to Estrone. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and
incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Estrone in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
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人 前列腺素E1(PGE1) 酶联免疫分析（ ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆, 组织及相关液体样本中前列腺素E1(PGE1) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人前列腺素 E1(PGE1) 水平。用纯化的人前列腺素 E1(PGE1) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入前列腺素 E1
at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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