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- 详细信息
- 文献和实验
- 技术资料
- 库存:
货源充足
- 供应商:
LSM Bio
- 检测范围:
详见说明书
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测大鼠血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
大鼠
- 标记物:
详见说明书
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 规格:
96T
Rat Gamma Hydroxybutyric acid, GHB ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Gamma-Hydroxybutyric acid, 4-hydroxybutyric acid, GHB,GHBA
Search name
Rat GHB ELISA Kit, Rat GHB ELISA Kit, Rat Gamma 4-hydroxybutyric acid ELISA Kit, Rat GHBA ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Gamma-Hydroxybutyric acid concentrations in serum,
plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Gamma-Hydroxybutyric acid, During the reaction, Gamma-Hydroxybutyric acid in the sample or
standard competes with a fixed amount of biotin-labeled Gamma-Hydroxybutyric acid for sites on a pre-coated Monoclonal antibody
specific to Gamma-Hydroxybutyric acid. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Gamma-Hydroxybutyric acid in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
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文献和实验This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown here). A. PROTOCOL 1. prepare platelets
OUTLINE This modification of qualitative ELISA (Enzyme-Linked Immunosorbent Assay) is used for either screening detection of anti-platelet antibodies or for detection of platelet-associated Ig (PAIg) (shown here). PROTOCOL
Characterization of Wild‐Type Excitatory Amino Acid Ion Channel Receptors
. Influences of ions, enzymes and detergents on gamma‐aminobutyric acid‐receptor binding in synaptic membranes of rat brain. Mol. Pharmacol. 13:442‐453. Ferkany, J.W., and Coyle, J.T
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