Lucifer Yellow CH dilithium sa

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Lucifer Yellow CH dilithium salt

CAS No. : 67769-47-5

MCE 国际站：Lucifer Yellow CH dilithium salt

产品活性：Lucifer Yellow CH dilithium salt 是一种高强荧光探针，含有游离的肼基，可在室温下与脂肪醛发生反应。Lucifer Yellow CH 可作为生物示踪剂，用于监测神经元的分支、再生、间隙连接检测和表征，以及醛固定后选择性消融细胞。Lucifer yellow CH 的激发/发射最大值分别为 430/540 nm。

研究领域：Others

作用靶点：Fluorescent Dye

In Vitro: Preparation of Lucifer Yellow CH (dilithium salt) working solution
1 Preparation of the stock solution
Dissolve Lucifer Yellow CH (dilithium salt) in DDH₂O to obtain 1 mg/mL of Lucifer Yellow CH (dilithium salt).
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
2. Preparation of Lucifer Yellow CH (dilithium salt) working solution.
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 0.5% of Lucifer Yellow CH (dilithium salt) working solution.
Note: Please adjust the concentration of Lucifer Yellow CH (dilithium salt) working solution according to the actual situation.

Cell staining
1. Cell preparation:
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2. Add 1 mL of Lucifer Yellow CH (dilithium salt) working solution, and then incubate at room temperature for 5-30 minutes.
3. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
4. Wash twice with PBS, 5 minutes each time.
5. Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

Precautions
1. It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of Lucifer Yellow CH (dilithium salt) working solution according to the actual situation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.

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