Bovine SLC35A3 ELISA KIT ,Bovine AMRS ELISA KIT ,Bovine UDP-N-acetylglucosamine transporter ELISA KIT ,Bovine golgi UDP-GlcNAc transporter ELISA KIT ,Bovine solute carrier family 35 (UDP-N-acetylglucosamine (UDP-GlcNAc) transporter) member 3 ELISA KIT ,Bovine solute carrier family 35 (UDP-N-acetylglucosamine (UDP-GlcNAc) transporter) member A3 ELISA KIT ,Bovine solute carrier family 35 member A3 ELISA KIT
Intended use
Bovine SLC35A3 ELISA kit allows for the in vitro quantitative determination of Bovine SLC35A3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
Bovine SLC35A3 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SLC35A3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SLC35A3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SLC35A3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.