Bovine PDE5A ELISA KIT allows for the in vitro quantitative determination of Bovine cgmp-specific 3',5'-cyclic phosphodiesterase,PDE5A concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Bovine PDE5A ELISA KIT has been pre-coated with an antibody specific to PDE5A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PDE5A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PDE5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PDE5A in the samples is then determined by comparing the O.D. of the samples to the standard curve.