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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Glutamine-rich protein 1,QRICH1
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Glutamine- rich protein 1, QRICH1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
QRICH1,glutamine-rich protein 1; glutamine-rich 1
Search name
Bovine QRICH1 ELISA KIT, Bovine glutamine-rich protein 1 ELISA KIT, Bovine glutamine-rich 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine Glutamine- rich protein 1, QRICH1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to QRICH1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for QRICH1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain QRICH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of QRICH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
Procedures for In Vitro Production of Bovine Embryos
, Chenoweth PJ, Hansen PJ. Deleterious actions of gossypol on bovine spermatozoa, oocytes and embryos. Biol Reprod 1997; 57, 901-907.Edwards JL, Ealy AD, Monterroso VH, Hansen PJ. Ontogeny of temperature-regulated heat shock protein 70 synthesis
Solid Phase Assays for Studying ECM Protein-Protein Interactions
of an enzyme-linked immunosorbent assay (ELISA) plate (solid phase). The plate is then blocked using bovine serum albumin (BSA), and biotin-labeled protein B (here termed ’ligand’) is added. After washing the wells to remove unbound ligand, bound ligand
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