Bovine UTP- - glucose- 1- phosphate uridylyltransferase, UGP2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
UGP2,UDPG; UDPGP; UDPGP2; UGP1; UGPP1; UGPP2; pHC379; UDP-glucose diphosphorylase; UDP-glucose pyrophosphorylase 1; UGPase 2; UTP--glucose-1-phosphate uridylyltransferase; UTP--glucose-1-phosphate uridylyltransferase 2; UTP-glucose-1-phosphate uridyltransferase; Uridyl diphosphate glucose pyrophosphorylase-1; uridyl diphosphate glucose pyrophosphorylase 2; UDP-glucose pyrophosphorylase 2
Search name
Bovine UGP2 ELIAS KIT,Bovine UDPG ELIAS KIT,Bovine UDPGP ELIAS KIT,Bovine UDPGP2 ELIAS KIT,Bovine UGP1 ELIAS KIT,Bovine UGPP1 ELIAS KIT,Bovine UGPP2 ELIAS KIT,Bovine pHC379 ELIAS KIT,Bovine UDP-glucose diphosphorylase ELIAS KIT,Bovine UDP-glucose pyrophosphorylase 1 ELIAS KIT,Bovine UGPase 2 ELIAS KIT,Bovine UTP--glucose-1-phosphate uridylyltransferase ELIAS KIT,Bovine UTP--glucose-1-phosphate uridylyltransferase 2 ELIAS KIT,Bovine UTP-glucose-1-phosphate uridyltransferase ELIAS KIT,Bovine Uridyl diphosphate glucose pyrophosphorylase-1 ELIAS KIT,Bovine uridyl diphosphate glucose pyrophosphorylase 2 ELIAS KIT,Bovine UDP-glucose pyrophosphorylase 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine UTP- - glucose- 1- phosphate uridylyltransferase, UGP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP-glucose pyrophosphorylase, During the reaction, UDP-glucose pyrophosphorylase in the sample or standard competes with a fixed amount of biotin-labeled UDP-glucose pyrophosphorylase for sites on a pre-coated Monoclonal antibody specific to UDP-glucose pyrophosphorylase. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UDP-glucose pyrophosphorylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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