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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine DnaJ homolog subfamily C member 5,DNAJC5
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine DnaJ homolog subfamily C member 5, DNAJC5 ELISA KIT
Product Name:Bovine DnaJ homolog subfamily C member 5, DNAJC5 ELISA KIT
Packing:96T
Catalog No.:ELI-47797b
Gene Name:Bovine DnaJ homolog subfamily C member 5, DNAJC5
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Bovine DnaJ homolog subfamily C member 5, DNAJC5
Alternative Name:DNAJC5,Bovine DnaJ homolog subfamily C member 5, DNAJC5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine DnaJ homolog subfamily C member 5, DNAJC5 ELISA KIT allows for the in vitro quantitative determination of Bovine DnaJ homolog subfamily C member 5, DNAJC5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine DnaJ homolog subfamily C member 5, DNAJC5 ELISA KIT has been pre-coated with an Bovine DnaJ homolog subfamily C member 5, DNAJC5 antibody specific to Bovine DnaJ homolog subfamily C member 5, DNAJC5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine DnaJ homolog subfamily C member 5, DNAJC5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine DnaJ homolog subfamily C member 5, DNAJC5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine DnaJ homolog subfamily C member 5, DNAJC5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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Preparation 1. DNeasy Plant Mini Kit (Qiagen) (see Note 1). 2. Cells or tissue from which DNA will be extracted. 3. Proteinase K digestion: 50 ug/ml Proteinase K in 100 ug/mlin 0.01 M Tris–HCl, pH 7.8, 5 mM EDTA, 0.5% SDS. 4. Saturated
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