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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Nuclear cap-binding protein subunit 2,NCBP2
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Nuclear cap- binding protein subunit 2, NCBP2 ELISA KIT
Product Name:Bovine Nuclear cap- binding protein subunit 2, NCBP2 ELISA KIT
Packing:96T
Catalog No.:ELI-42538b
Gene Name:Bovine Nuclear cap-binding protein subunit 2, NCBP2
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Bovine Nuclear cap-binding protein subunit 2, NCBP2
Alternative Name:NCBP2,Bovine Nuclear cap-binding protein subunit 2, NCBP2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Nuclear cap- binding protein subunit 2, NCBP2 ELISA KIT allows for the in vitro quantitative determination of Bovine Nuclear cap-binding protein subunit 2, NCBP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Nuclear cap- binding protein subunit 2, NCBP2 ELISA KIT has been pre-coated with an Bovine Nuclear cap-binding protein subunit 2, NCBP2 antibody specific to Bovine Nuclear cap-binding protein subunit 2, NCBP2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Nuclear cap-binding protein subunit 2, NCBP2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Nuclear cap-binding protein subunit 2, NCBP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Nuclear cap-binding protein subunit 2, NCBP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
for mapping distributions of chromosome-associated proteins, identifying and characterizing cis -regulatory DNA elements bound by particular proteins, and mapping functional protein domains necessary for chromosomal binding or other activities
Protein‐Protein Interactions Identified by Pull‐Down Experiments and Mass Spectrometry
spectrometry, pull?downs can be considered as the protein?based equivalent of a yeast two?hybrid screen. To improve the isolation of specific binding partners, pull?down methods are described involving the use of cross?linking, large?scale tissue lysates
Purification of Sequence‐Specific DNA‐Binding Proteins by Affinity Chromatography
Gander, I., Foeckler, R., Rogge, L., Meisterernst, M., Schneider, R., Mertz, R., Lottspeich, F., and Winnacker, E.L. 1988. Purification methods for the sequence‐specific DNA‐binding protein nuclear factor I (NFI)—generation of protein
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