Bovine Galactoside 3(4)- L- fucosyltransferase, FUT3 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
FUT3 CD174; FT3B; FucT-III; Les; Lewis FT; alpha-(1,3/1,4)-fucosyltransferase; blood group Lewis alpha-4-fucosyltransferase; fucosyltransferase III; galactoside 3(4)-L-fucosyltransferase; fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group),Galactoside 3(4)-L-fucosyltransferase,Fucosyltransferase III(FucT-III), Blood group Lewis alpha-4-fucosyltransferase (Lewis FT), Fucosyltransferase 3,;
Search name
Bovine FUT3 ELISA KIT ,Bovine CD174 ELISA KIT ,Bovine FT3B ELISA KIT ,Bovine FucT-III ELISA KIT ,Bovine Les ELISA KIT ,Bovine Lewis FT ELISA KIT ,Bovine fucosyltransferase III ELISA KIT ,Bovine galactoside 3(4)-L-fucosyltransferase ELISA KIT ,Bovine fucosyltransferase 3 ELISA KIT ,Bovine galactoside 3(4)-L-fucosyltransferase Lewis blood group ELISA KIT ,Bovine Galactoside 3(4)-L-fucosyltransferase ELISA KIT ,Bovine Fucosyltransferase III ELISA KIT ,Bovine blood group Lewis alpha-4-fucosyltransferase ELISA KIT,Bovine alpha-(1,3/1,4)-fucosyltransferase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine Galactoside 3(4)-L-fucosyltransferase, FUT3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to FUT3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FUT3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FUT3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FUT3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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