- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-29722b
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Serine/threonine-protein kinase Sgk1,SGK1
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Serine/threonine- protein kinase Sgk1, SGK1 ELISA KIT
Product Name:Bovine Serine/threonine- protein kinase Sgk1, SGK1 ELISA KIT
Packing:96T
Catalog No.:ELI-29722b
Gene Name:Bovine Serine(threonine-protein kinase Sgk1, SGK1
Detect Range:3.125-200ng/ml
Sensitivity:1.875ng/ml
Target Protein Name:Bovine Serine(threonine-protein kinase Sgk1, SGK1
Alternative Name:SGK1,Bovine Serine(threonine-protein kinase Sgk1, SGK1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Serine/threonine- protein kinase Sgk1, SGK1 ELISA KIT allows for the in vitro quantitative determination of Bovine Serine(threonine-protein kinase Sgk1, SGK1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Serine/threonine- protein kinase Sgk1, SGK1 ELISA KIT has been pre-coated with an Bovine Serine(threonine-protein kinase Sgk1, SGK1 antibody specific to Bovine Serine(threonine-protein kinase Sgk1, SGK1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Serine(threonine-protein kinase Sgk1, SGK1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Serine(threonine-protein kinase Sgk1, SGK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Serine(threonine-protein kinase Sgk1, SGK1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验An In Vitro Assay for the Kinase Activity of mTOR Complex 2
The mTOR complex 2 (mTORC2) is a protein kinase complex involved in many important physiological processes through the regulation of its substrates, such as Akt, SGK1, and conventional PKC. Its activity is modulated negatively by interaction
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
.C., Asara, J.M., Lane, W.S., Garner, C.C., and Lienhard, G.E. 2002. A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase‐activating protein (GAP) domain. J. Biol. Chem. 277:22115‐22118
. 1. Introduction Glycogen synthase kinase (GSK)-3 is a serine/threonine proteinkinase which was first described as a component of insulin-mediatedsignaling pathway (1) . There are two homologous mammalianisoforms, encoded by different genes, GSK-3a and GSK
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