Bovine Interferon alpha- D, IFNAD ELISA KIT
Product Name:Bovine Interferon alpha- D, IFNAD ELISA KIT
Packing:96T
Catalog No.:ELI-07904b
Gene Name:Bovine Interferon alpha-D, IFNAD
Detect Range:15.625-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Bovine Interferon alpha-D, IFNAD
Alternative Name:IFNAD,Bovine Interferon alpha-D, IFNAD
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Interferon alpha- D, IFNAD ELISA KIT allows for the in vitro quantitative determination of Bovine Interferon alpha-D, IFNAD concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Interferon alpha- D, IFNAD ELISA KIT has been pre-coated with an Bovine Interferon alpha-D, IFNAD antibody specific to Bovine Interferon alpha-D, IFNAD .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Interferon alpha-D, IFNAD and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Interferon alpha-D, IFNAD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Interferon alpha-D, IFNAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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