- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-06416b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Apolipoprotein C-II,APOC2
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Apolipoprotein C-II, APOC2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
APOC2,APO-CII; APOC-II; Apo-CII, apolipoprotein C2; apolipoprotein C-II
Search name
Bovine APOC2 ELISA KIT ,Bovine APO-CII ELISA KIT ,Bovine APOC-II ELISA KIT ,Bovine Apo-CII ELISA KIT ,Bovine apolipoprotein C2 ELISA KIT ,Bovine apolipoprotein C-II ELISA KIT
Intended use
Bovine APOC2 ELISA KIT allows for the in vitro quantitative determination of bovine apolipoprotein c-ii,Apo-CII concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Bovine APOC2 ELISA KIT has been pre-coated with an antibody specific to Apo-CII. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Apo-CII and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Apo-CII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Apo-CII in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Bovine APOC2 ELISA KIT Range 1.56-100ng/ml
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of ice-cold PBS containing protease inhibitor cocktail. 5. Prepare 0.2X trypsin diluted in PBS, and add 5 mL of it to the cells. Incubate in 5% CO2 for 5 min at 37°C. 6. Neutralize the trypsin with 10 mL of 10% fetal bovine
DETECTION OF RECOMBINANT PROTEINS BY ELISA
0.5 g BSA QS to 500 ml with H 2O. ELISA Conjugate Diluent PBS, 0.05% Tween-20, 0.1% BSA, 2 µg/ml IgG) Make above ELISA diluent and add 1 mg/ml Bovine Cohn Fraction II IgG
V294.PART II，Chapter 5 细胞粘附实验（英文版）
by centrifugation in a microfuge at 3800g for 15 min and then keepthe cell pellets in –20°C freezer. Simultaneous to step 7, add 600 μL of the substratesolution to the microtube, vortex, and then transfer the cell/substrate mixtureto new wells of the 96-well ELISA
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