Bovine OPRM1 ELISA KIT allows for the in vitro quantitative determination of Bovine mu-type opioid receptor,M-OR-1 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Bovine OPRM1 ELISA KIT has been pre-coated with an antibody specific to M-OR-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for M-OR-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain M-OR-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of M-OR-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.