- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELA-E0448b
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Insulin
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Insulin, INS ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Insulin,INS, IDDM2; ILPR; IRDN; MODY10; preproinsulin; proinsulin
Search name
Bovine Insulin ELISA Kit, Bovine INS ELISA Kit, Bovine IDDM2 ELISA Kit, Bovine ILPR ELISA Kit, Bovine MODY10 ELISA Kit,
Bovine preproinsulin ELISA Kit, Bovine proinsulin ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine insulin,Insulin concentrations in serum, plasma, tissue
homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Insulin, During the reaction, Insulin in the sample or standard competes with a fixed amount of
biotin-labeled Insulin for sites on a pre-coated Monoclonal antibody specific to Insulin. Excess conjugate and unbound sample or standard
are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Insulin in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验结晶牛胰岛素（crystallized bovine insulin）
牛胰岛素的晶状体。牛胰岛素是牛胰脏中胰岛β-细胞所分泌的一种调节糖代谢的蛋白质激素。其一级结构1955年由英国桑格（S.Sanger）测定。我国中科院生化研究所、北大化学系及中科院有机化学研究所通力合作，于1959年开始工作、1965年9月获得了用人工方法合成的、有生物活性的结晶牛胰岛素，实现了世界上首次人工合成蛋白质的壮举。
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
TUNEL PROCEDURE IN BOVINE EMBRYOS
. Dilute 1 µl RNase A (stock) with 999 µl PBS/PVP and then dilute this solution 1:1 with PBS/PVP to obtain a working solution (Use fresh). Mounting medium and antifade: ProLong Antifade Kit (Molecular
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