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- 详细信息
- 文献和实验
- 技术资料
- 库存：
货源充足
- 供应商：
LSM Bio
- 检测范围：
详见说明书
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测小鼠血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
牛
- 标记物：
详见说明书
- 样本：
血清、血浆或其它相关生物液体中天然和重组蛋白
- 规格：
96T
Bovine Prostaglandin E2 , PGE2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Prostaglandin E2,dinoprostone,
Search name
Bovine Prostaglandin E2 ELISA Kit, Prostaglandin E2 ELISA Kit, dinoprostone ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Prostaglandin E2 concentrations in serum,plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Prostaglandin E2, During the reaction, Prostaglandin E2 in the sample or standard competes with
a fixed amount of biotin-labeled Prostaglandin E2 for sites on a pre-coated Monoclonal antibody specific to Prostaglandin E2. Excess
conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is
added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is
terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450
nm ± 2 nm. The concentration of Prostaglandin E2 in the samples is then determined by comparing the O.D. of the samples to the
standard curve.
PDF Manual download
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文献和实验, such as prostaglandins (PG) and histamine, also affect Th1/Th2 cytokine production and Th differentiation (2 –7 ). PGE2 is one of prostanoids produced by action of the enzyme cyclooxygenase (COX) on arachidonic acid. PGE2 is mainly produced by APC: monocytes
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人前列腺素E2 ( PGE2 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液等生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 PGE2 单抗包被于酶标板上，标准品和样品中的 PGE2与单抗结合，加入生物素化的抗人 PGE2
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 兔前列腺素E2 ( PGE2 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗兔 PGE2 单抗包被于酶标板上，标准品和样品中的 PGE2与单抗结合，加入生物素化的抗兔 PGE2 ，形成
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