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- Assay Biotech
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- 美国
- 2026年03月31日
- 仓鼠
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| Product Name | 14-3-3 beta/zeta (Phospho-Ser184/186) Antibody |
| Synonyms | 14-3-3 protein zeta/delta; 1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1; Mitochondrial import stimulation factor S1 subunit; Protein kinase C inhibitor protein-1; YWHAZ |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 14-3-3 beta/zeta around the phosphorylation site of Ser184/186. |
| Immunogen Region | Internal |
| Clonality | Polyclonal |
| Modification sites | H:S184/186 M:S184/186 R:S184/186 |
| Application dilutions | WB: 1:500~1:1000 ELISA: 1:5000 |
| Specificity | 14-3-3 beta/zeta (Phospho-Ser184/186) Antibody detects endogenous levels of 14-3-3 beta/zeta only when phosphorylated at serine184/186. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
| Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration | 1 mg/ml |
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文献和实验, a small but significant proportion of 14-3-3 in brain is tightly and selectively associated with some membranes. Using a panel of isoform-specific antisera we find that the epsilon, eta, gamma, beta, and zeta isoforms are all present in purified synaptic
Introduction of human 14-3-3 proteins
-3-3 proteins are cytosolic,a small but significant proportion of 14-3-3 in brain is tightly and selectively associated with some membranes.Using a panel of isoform-specific antisera we find that the epsilon,eta,gamma,beta,and zeta isoforms are all present
下调不能说明活性降低，因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外，还要做磷酸化的GSK3-beta的蛋白表达。 （2）应该活性形式和非活性形式都做。一般来讲，GSK3-beta在Ser9位点磷酸化之后活性收到抑制，而在216位点磷酸化之后，其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了，另外还要同时检测GSK3-beta的总蛋白表达，这样才能全面的说明问题。
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