产品货号:jlcR3072 英文名称 :phospho-Afadin (Ser1721) 中文名称 :磷酸化丝状肌动蛋白结合蛋白抗体 别 名 :p-Afadin(Ser1721); Afadin (phospho S1721); Afadin (phospho Ser1721); MLLT4; Protein AF6; AF6; Afadin; AFAD_HUMAN; ALL1-fused gene from chromosome 6 protein; Protein AF-6. 产品类型 :磷酸化抗体 研究领域 :细胞生物 免疫学 神经生物学 信号转导 细胞凋亡 抗体来源 :Rabbit 克隆类型 :Polyclonal 交叉反应 : Human, Mouse, Rat, 产品应用 :WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分 子 量 :207kDa 细胞定位 :细胞膜 性 状 :Lyophilized or Liquid 浓 度 :1mg/ml 免 疫 原 :KLH conjugated Synthesised phosphopeptide derived from human Afadin around the phosphorylation site of Ser1721:VL(p-S)PD- 亚 型 :IgG 纯化方法 :affinity purified by Protein A 储 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 :Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. PubMed :PubMed 产品介绍 : Afadin is a cell-cell adherens junction F-actin binding multidomain protein. Two splice variants of afadin have been described: l-afadin, which is ubiquitously expressed, and the smaller s-afadin, which is abundantly expressed in neural tissue. l-Afadin contains one PDZ domain near its middle followed by three proline-rich and one F-actin-binding region at the carboxyl-terminal. l/s-Afadin is localized to cell-cell junctions and binds several cytoplasmic proteins such as the small GTPase Ras, the tight junction protein ZO-1, and the vinculin binding protein ponsin/SH3P12.
Function: Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
Subunit: Homodimer. Interacts with F-actin, nectin and PVRL3/nectin-3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1 and RIT2 (By similarity). Interacts with NRXN1 and BCR.
Subcellular Location: Cell junction, adherens junction. Note=Not found at cell-matrix AJs.
DISEASE: Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.