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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Hcn2| Mouse Potassium
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Hcn2 ELISA Kit
Catalog EF013261
Packing 48Tests/96Tests
ELISA Method Sandwich ELISA Species Mouse
Sample Mouse serum, plasma, tissue homogenates and other biological fluids and Recombinant Hcn2
Detect Range 0.312-20ng/ml sensitivity 0.188ng/ml
ELISA Intend Use
Mouse Hcn2 ELISA Kit is a Sandwich ELISA KIT detecting the concentration of Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 in Mouse serum, plasma, tissue homogenates and other biological fluids.
(ELISA KIT is only for Research Only, not for the diagnostic Use!)
:
:
: 3128136151
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Cellular ELISA ProtocolFormalin Fixed Cell Plates1. Trypsinize confluent flasks2. Pool and count cells3. Centrifuge at 1500 rpm for 10 minutes4. Resuspend to the appropriate concentration in complete medium4 x 105 cells/ml for epithelial cells2 x
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