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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Macrophage Inflammatory Protein-1 Alpha, MIP-1 Alpha ELI
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Macrophage Inflammatory Protein- 1 Alpha, MIP- 1 Alpha ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
C-C motif chemokine 3,TY-5,Small-inducible cytokine A3,SIS-alpha,Macrophage inflammatory protein 1-alpha(MIP-1-alpha),Heparin-binding chemotaxis protein,L2G25B, C-C motif chemokine 3,Tonsillar lymphocyte LD78 alpha protein,Small-inducible cytokine A3,SIS-beta,PAT 464.1,G0/G1 switch regulatory protein 19-1,Macrophage inflammatory protein 1-alpha,MIP-1-alpha,CCL3,G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3; C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; PAT 464.1; SIS-beta; macrophage inflammatory protein 1-alpha; small inducible cytokine A3 (homologous to mouse Mip-1a); tonsillar lymphocyte LD78 alpha protein; chemokine (C-C motif) ligand 3
Search name
Mouse C-C motif chemokine 3 ELISA KIT ,Mouse Tonsillar lymphocyte LD78 alpha protein ELISA KIT ,Mouse Small-inducible cytokine A3 ELISA KIT ,Mouse SIS-beta ELISA KIT ,Mouse PAT 464.1 ELISA KIT ,Mouse G0/G1 switch regulatory protein 19-1 ELISA KIT ,Mouse Macrophage inflammatory protein 1-alpha ELISA KIT ,Mouse MIP-1-alpha ELISA KIT ,Mouse CCL3 ELISA KIT ,Mouse G0S19-1 ELISA KIT ,Mouse LD78ALPHA ELISA KIT ,Mouse MIP-1-alpha ELISA KIT ,Mouse MIP1A ELISA KIT ,Mouse SCYA3 ELISA KIT ,Mouse C-C motif chemokine 3 ELISA KIT ,Mouse G0/G1 switch regulatory protein 19-1 ELISA KIT ,Mouse PAT 464.1 ELISA KIT ,Mouse SIS-beta ELISA KIT ,Mouse macrophage inflammatory protein 1-alpha ELISA KIT ,Mouse small inducible cytokine A3 (homologous to mouse Mip-1a) ELISA KIT ,Mouse tonsillar lymphocyte LD78 alpha protein ELISA KIT ,Mouse chemokine (C-C motif) ligand 3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse C-C motif chemokine 3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-C motif chemokine 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C-C motif chemokine 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C-C motif chemokine 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of C-C motif chemokine 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验References 1. R. M. O’Connell, K. D. Taganov, M. P. Boldin, G. Cheng, D. Baltimore, MicroRNA-155 is induced during the macrophage inflammatory response. Proc. Natl. Acad. Sci. U.S.A. 104, 1604–1609 (2007).[Abstract/Free Full Text] 2. D
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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