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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
von Willebrand factor A domain-containing protein 8, VWA8
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse von Willebrand factor A domain-containing protein 8, VWA8 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
VWA8,KIAA0564; von Willebrand factor A domain-containing protein 8; von Willebrand factor A domain containing 8, Uncharacterized protein KIAA0564
Search name
Mouse VWA8 ELISA KIT ,Mouse KIAA0564 ELISA KIT ,Mouse von Willebrand factor A domain-containing protein 8 ELISA KIT ,Mouse von Willebrand factor A domain containing 8 ELISA KIT ,Mouse Uncharacterized protein KIAA0564 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse von Willebrand factor A domain-containing protein 8, VWA8 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to VWA8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for VWA8 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain VWA8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VWA8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
:
:
: 3128136151
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文献和实验was unresponsive to the transcription factors (Fig. 4D). The sequence 5'-GGAA-3' (ETS-binding site-2, EBS2) is conserved between mouse and the human promoters, and in the mouse it interacts with the ETS-1 protein, a transcription factor of the ETS family expressed
Studies of the Ubiquitin Proteasome System
Basic Protocol 9: Substrate Ubiquitylation by Multisubunit E3s Materials VBC‐Cul2 E3 protein complex expressed and purified using baculoviral expression system ( protocol 45 ) Mouse E1
isolation kit, Qiagen), pooled mRNA for each treatment group. As well as GAPDH, factor VII, glucose-6-phosphatase and VEGF mRNAs were also used for normalization. ELISA was used to quantify the reduction of apoB-100 protein levels in mouse plasma
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