Interleukin-18, IL-18 ELISA Ki

Mouse Interleukin-18, IL-18 ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Interleukin-18(IL-18),;Interferon gamma-inducing factor(IFN-gamma-inducing factor),Interleukin-1 gamma(IL-1 gamma),; IGIF; IL-1g;
IL1F4; iboctadekin;

Search name
Mouse Interleukin- 18 ELISA Kit, Mouse IL- 18 ELISA Kit, Mouse Interleukin 18 ELISA Kit, Mouse IL18 ELISA Kit, Mouse
Interferon gamma-inducing factor ELISA Kit, Mouse IFN-gamma-inducing factor ELISA Kit, Mouse Interleukin-1 gamma ELISA
Kit, Mouse IL-1 gamma ELISA Kit, Mouse IGIF ELISA Kit, Mouse IL-1g ELISA Kit, Mouse IL1F4 ELISA Kit, Mouse iboctadekin
ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse interleukin-18,IL-18 concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-18. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-18 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain IL-18, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-18 in the samples is then determined by comparing the
O.D. of the samples to the standard curve.

PDF manual download

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