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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Sorting nexin-25, Snx25
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Sorting nexin- 25, Snx25 ELISA KIT
Product Name:Mouse Sorting nexin- 25, Snx25 ELISA KIT
Packing:96T
Catalog No.:ELI-53288m
Gene Name:Mouse Snx25
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Snx25
Alternative Name:Mouse Sorting nexin-25, Snx25
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Sorting nexin- 25, Snx25 ELISA KIT allows for the in vitro quantitative determination of Mouse Snx25 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Sorting nexin- 25, Snx25 ELISA KIT has been pre-coated with an Mouse Sorting nexin-25, Snx25 antibody specific to Mouse Snx25 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Snx25 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Snx25 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Snx25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Cellular ELISA ProtocolFormalin Fixed Cell Plates1. Trypsinize confluent flasks2. Pool and count cells3. Centrifuge at 1500 rpm for 10 minutes4. Resuspend to the appropriate concentration in complete medium4 x 105 cells/ml for epithelial cells2 x
A. Formalin Fixed Cell Plates 1. Trypsinize confluent flasks 2. Pool and count cells 3. Centrifuge at 1500 rpm for 10 minutes 4. Resuspend to the appropriate concentration in complete medium4 x 105 cells/ml for epithelial cells
. 7. Wash 3X with DFH and 2X with HBSS. 8. Add 50 ml/well working substrate solution 0.5 ml 4.0% OPD 50 ml 30% H2O2 1.0 ml 10X Substrate buffer 8.5 ml di-H2O. 9. Incubate for 20 minutes at room temperature. 10. Add 25 ml/well 4.5N Sulfuric Acid
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