Mouse myelin-associated glycop

Mouse myelin- associated glycoprotein, MAG ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MAG,myelin associated glycoprotein,S-MAG; GMA; SIGLEC-4A; SIGLEC4A; sialic acid binding Ig-like lectin 4A; sialic acid-binding immunoglobulin-like lectin 4A, Myelin-associated glycoprotein,

Search name

Mouse MAG ELISA KIT ,Mouse myelin associated glycoprotein ELISA KIT ,Mouse S-MAG ELISA KIT ,Mouse GMA ELISA KIT ,Mouse SIGLEC-4A ELISA KIT ,Mouse SIGLEC4A ELISA KIT ,Mouse sialic acid binding Ig-like lectin 4A ELISA KIT ,Mouse sialic acid-binding immunoglobulin-like lectin 4A ELISA KIT ,Mouse Myelin-associated glycoprotein ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse Myelin-associated glycoprotein concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myelin-associated glycoprotein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Myelin-associated glycoprotein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Myelin-associated glycoprotein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Myelin-associated glycoprotein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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