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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
40S ribosomal protein S15a, Rps15a
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse 40S ribosomal protein S15a, Rps15a ELISA KIT
Product Name:Mouse 40S ribosomal protein S15a, Rps15a ELISA KIT
Packing:96T
Catalog No.:ELI-52745m
Gene Name:Mouse Rps15a
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Rps15a
Alternative Name:Mouse 40S ribosomal protein S15a, Rps15a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse 40S ribosomal protein S15a, Rps15a ELISA KIT allows for the in vitro quantitative determination of Mouse Rps15a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse 40S ribosomal protein S15a, Rps15a ELISA KIT has been pre-coated with an Mouse 40S ribosomal protein S15a, Rps15a antibody specific to Mouse Rps15a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rps15a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rps15a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rps15a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验明确的蛋白质如下述: S1 :与蛋白质合成的 i因子(干扰因子)和 Qβ复制酶的亚基Ⅰ为同一物质,可与 mRNA结合; S4 : ram(核糖体的双关性 ribosomal ambiguity)基因的产物; S5 : SPc〔壮观霉素( Spectinomycin)抗性〕基因的产物; S12 : str(链霉素抗性)基因的产物; L7 、 L12 :有和多肽链延长因子 Tu及 G间的相互作用,也有和起始因子和终止因子的相互作用。 L11 :肽基转移酶。
缩写为 rRNA。是构成核糖体的 RNA,约占细胞总 RNA的 80%。真核细胞的核糖体 60S亚基含三种 RNA;即 28SRNA(分子量 1.6× 106 )、 7SRNA(或 5.7S)和 5SRNA(分子量 4× 104 )含各一分子中。 40S亚基与此相反仅含 1分子 18SRNA(分子量 0.7× 106 )。原核细胞中 50S亚基含 23SRNA(分子量 1.2× 106 )和 5S, RNA(分子量 4× 104 ), 30S亚基含 16SRNA
核糖体循环 ribosomal cycle ribosome-polysome cycle
指在细胞内构成核糖体的大小两种亚单位(沉淀系数为50S或 60S的大亚单位和 30S或 40S的小亚单位)与蛋白活体合成开始会合( 70S或 80S粒子形成),合成后又分离的这一反复循环而言(参见核糖体)。核糖体在不合成蛋白时,分离成亚单位,这是由于多肽链起始因子之一与小亚单位结合,而抑制了与大亚单位的结合。这种状态的小亚单位,如果与其它起始因子、起始 tRNA、 mRNA结合,则形成多肽链起始复合体。随着与大亚单位结合,在多肽链延长因子存在下进行多肽链延长反应。多数的核糖体在一分
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