Proline-rich protein 5-like, P
rr5l ELISA KIT/ Mouse Proline-rich protein 5-like, Prr5l ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-52386m
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Proline-rich protein 5-like, Prr5l
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Proline- rich protein 5- like, Prr5l ELISA KIT
Product Name:Mouse Proline- rich protein 5- like, Prr5l ELISA KIT
Packing:96T
Catalog No.:ELI-52386m
Gene Name:Mouse Prr5l
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Prr5l
Alternative Name:Mouse Proline-rich protein 5-like, Prr5l
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Proline- rich protein 5- like, Prr5l ELISA KIT allows for the in vitro quantitative determination of Mouse Prr5l concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Proline- rich protein 5- like, Prr5l ELISA KIT has been pre-coated with an Mouse Proline-rich protein 5-like, Prr5l antibody specific to Mouse Prr5l .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Prr5l and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Prr5l , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Prr5l in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验玉米Proline responding 1(pro1)突变在蛋白合成和细胞周期调控中起到关键作用
上海大学生命科学学院、上海市能源作物育种及应用重点实验室的研究人员证实，玉米Pro1基因(Zm P5CS2)的突变造成了突变体细胞中脯氨酸(proline)合成受阻，从而导致proline积累的减少。突变体中proline的缺乏引起了相应的转运RNA(tRNApro AGG)空载形式(uncharged tRNApro AGG)的增多，从而激活了细胞中的GCN2激酶，该激酶通过磷酸化真核生物翻译起始因子eIF2α从而抑制了细胞中蛋白合成的普遍抑制；另一方面，突变体中proline的缺乏也引起
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
Protocol for Protein Extraction
Protocol for Protein Extraction 10 % w/v TCA/ acetone/ 0.07 % v/v -Mercaptoethanol Plant cells are rich in compounds that interfere with the 2DE separation method such as salts, organic acids, phenolics, pigments, terpenes
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