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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
DNA repair protein RAD51 homolog 3, Rad51c
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse DNA repair protein RAD51 homolog 3, Rad51c ELISA KIT
Product Name:Mouse DNA repair protein RAD51 homolog 3, Rad51c ELISA KIT
Packing:96T
Catalog No.:ELI-52284m
Gene Name:Mouse Rad51c
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Rad51c
Alternative Name:Mouse DNA repair protein RAD51 homolog 3, Rad51c
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DNA repair protein RAD51 homolog 3, Rad51c ELISA KIT allows for the in vitro quantitative determination of Mouse Rad51c concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DNA repair protein RAD51 homolog 3, Rad51c ELISA KIT has been pre-coated with an Mouse DNA repair protein RAD51 homolog 3, Rad51c antibody specific to Mouse Rad51c .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rad51c and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rad51c , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rad51c in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验several assays for detecting several kinds of DNA damage (strand breaks, internal crosslinking, DNA/protein crosslinks) and repair activity following exposure to genotoxic agents. The methods include single?cell electrophoresis (comet assay), filter eluting, K
. The second stepinvolves DNA repair process activated by the targeted cleavage,carried out normally by the endogenous DNA damage repair machinery within the cells. In this step, two major pathways couldbe employed, achieving different types of genome modifi
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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