Ubiquitin carboxyl-terminal hy
drolase 30, Usp30 ELISA KIT/ Mouse Ubiquitin carboxyl-terminal hydrolase 30, Usp30 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-51892m
- 2025年12月25日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Ubiquitin carboxyl-terminal hydrolase 30, Usp30
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Ubiquitin carboxyl- terminal hydrolase 30, Usp30 ELISA KIT
Product Name:Mouse Ubiquitin carboxyl- terminal hydrolase 30, Usp30 ELISA KIT
Packing:96T
Catalog No.:ELI-51892m
Gene Name:Mouse Usp30
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Usp30
Alternative Name:Mouse Ubiquitin carboxyl-terminal hydrolase 30, Usp30
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ubiquitin carboxyl- terminal hydrolase 30, Usp30 ELISA KIT allows for the in vitro quantitative determination of Mouse Usp30 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ubiquitin carboxyl- terminal hydrolase 30, Usp30 ELISA KIT has been pre-coated with an Mouse Ubiquitin carboxyl-terminal hydrolase 30, Usp30 antibody specific to Mouse Usp30 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Usp30 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Usp30 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Usp30 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
A Method for Assaying Deubiquitinating Enzymes
constants (kc/Km ) for the hydrolysis of Ub-AMC are 104 - and 107 -fold over those for the cleavage of Cbz-LRGG-AMC for isopeptidase T andUCH-L3 respectively. UCH--L3 is a 26 kDa Ub C-terminal hydrolase (30 ) isolated from rabbit reticulocytes
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
. For cytospin slides : i) fix air dried cell samples with a freshly prepared paraformaldeyde solution (4% in PBS, pH 7.4) for 30 min at room temperature; ii) rinse slides with PBS and incubate in permeabilisation solution (0.1% Triton X-100, 0.1% sodium citrate
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