NEDD8-conjugating enzyme UBE2F

Mouse NEDD8- conjugating enzyme UBE2F, Ube2f ELISA KIT

Product Name:Mouse NEDD8- conjugating enzyme UBE2F, Ube2f ELISA KIT
Packing:96T

Catalog No.:ELI-51875m

Gene Name:Mouse Ube2f

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse Ube2f

Alternative Name:Mouse NEDD8-conjugating enzyme UBE2F, Ube2f
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse NEDD8- conjugating enzyme UBE2F, Ube2f ELISA KIT allows for the in vitro quantitative determination of Mouse Ube2f concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse NEDD8- conjugating enzyme UBE2F, Ube2f ELISA KIT has been pre-coated with an Mouse NEDD8-conjugating enzyme UBE2F, Ube2f antibody specific to Mouse Ube2f .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ube2f and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ube2f , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ube2f in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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