Tryptase gamma, Tpsg1 ELISA KI

Mouse Tryptase gamma, TPSG1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Tryptase gamma,Serine protease 31,Transmembrane tryptase, TPSG1,PRSS31; TMT; trpA; gamma I; gamma II; lung tryptase; mast cell protease II; mast cell tryptase; pituitary tryptase; serine protease 31; skin tryptase; transmembrane tryptase; tryptase gamma; tryptase gamma I; tryptase gamma II; tryptase gamma 1

Search name

Mouse Tryptase gamma ELISA KIT ,Mouse Serine protease 31 ELISA KIT ,Mouse Transmembrane tryptase ELISA KIT ,Mouse  TPSG1 ELISA KIT ,Mouse PRSS31 ELISA KIT ,Mouse TMT ELISA KIT ,Mouse trpA ELISA KIT ,Mouse gamma I ELISA KIT ,Mouse gamma II ELISA KIT ,Mouse lung tryptase ELISA KIT ,Mouse mast cell protease II ELISA KIT ,Mouse mast cell tryptase ELISA KIT ,Mouse pituitary tryptase ELISA KIT ,Mouse serine protease 31 ELISA KIT ,Mouse skin tryptase ELISA KIT ,Mouse transmembrane tryptase ELISA KIT ,Mouse tryptase gamma ELISA KIT ,Mouse tryptase gamma I ELISA KIT ,Mouse tryptase gamma II ELISA KIT ,Mouse tryptase gamma 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Tryptase gamma concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptase gamma. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Tryptase gamma and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Tryptase gamma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Tryptase gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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