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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Zinc finger RNA-binding protein, Zfr
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Zinc finger RNA- binding protein, Zfr ELISA KIT
Product Name:Mouse Zinc finger RNA- binding protein, Zfr ELISA KIT
Packing:96T
Catalog No.:ELI-51558m
Gene Name:Mouse Zfr
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Zfr
Alternative Name:Mouse Zinc finger RNA-binding protein, Zfr
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Zinc finger RNA- binding protein, Zfr ELISA KIT allows for the in vitro quantitative determination of Mouse Zfr concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Zinc finger RNA- binding protein, Zfr ELISA KIT has been pre-coated with an Mouse Zinc finger RNA-binding protein, Zfr antibody specific to Mouse Zfr .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Zfr and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Zfr , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Zfr in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
. Cui, X., Ji, D., Fisher, D.A., Wu, Y., Briner, D.M., and Weinstein, E.J. 2011. Targeted integration in rat and mouse embryos with zinc‐finger nucleases. Nat. Biotechnol. 29:64‐67
Mol Cell:浙大王青青团队等合作揭示乳酸化调控 RNA m⁶A 修饰促进肿瘤浸润髓系细胞的免疫抑制功能
发生在 METTL3 蛋白的「CCCH」锌指结构域(zinc finger domain, ZFD),而 ZFD 起着 target recognition domain(TRD)的作用 [8],增强 METTL3 结合并催化靶 RNA 的 m6A 修饰。 本研究从「代谢-表观-转录」层面,揭示肿瘤微环境持续积累的乳酸如何促进 METTL3 表达和功能,继而以 m6A 修饰方式增强 JAK1-STAT3 枢纽信号活化,结果可能为靶向髓系细胞的肿瘤免疫治疗策略提供新的启示。
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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