- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-51482m
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Protein Wnt-7a, Wnt7a
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Protein Wnt- 7a, WNT7A ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Protein Wnt-7a, WNT7A,proto-oncogene Wnt7a protein; wingless-type MMTV integration site family member 7Aa
Search name
Mouse Protein Wnt-7a ELISA KIT ,Mouse WNT7A ELISA KIT ,Mouse proto-oncogene Wnt7a protein ELISA KIT ,Mouse wingless-type MMTV integration site family member 7Aa ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Protein Wnt-7a concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Wnt-7a. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Protein Wnt-7a and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein Wnt-7a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein Wnt-7a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
v468.chapter 1 经典 Wnt/b-Catenin 信号通路 （英文完整版）
, b-catenin , Groucho , Tcf , Target gene , Constitutive activation , Stem cell ,Colon cancer. 1. Introduction The Wnt pathway derives its name from the Drosophila (fruit-fly)W ingless gene and the mouse I NT -1 gene. The mouse Wnt1gene
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