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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Protein unc-45 homolog B, Unc45b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Protein unc- 45 homolog B, Unc45b ELISA KIT
Product Name:Mouse Protein unc- 45 homolog B, Unc45b ELISA KIT
Packing:96T
Catalog No.:ELI-51371m
Gene Name:Mouse Unc45b
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Unc45b
Alternative Name:Mouse Protein unc-45 homolog B, Unc45b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Protein unc- 45 homolog B, Unc45b ELISA KIT allows for the in vitro quantitative determination of Mouse Unc45b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Protein unc- 45 homolog B, Unc45b ELISA KIT has been pre-coated with an Mouse Protein unc-45 homolog B, Unc45b antibody specific to Mouse Unc45b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Unc45b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Unc45b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Unc45b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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: 3128136151
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文献和实验Determination of CD45 Tyrosine Phosphatase Activity in T Lymphocytes
CD45 is a leukocyte specific protein tyrosine phosphatase and is a major lymphocyte cell surface glycoprotein (see refs. 1 –3 for reviews). CD45 has been shown to be important for the transmission of signals by the T and B cell antigen
中的蛋白酪氨酸激酶。静止T细胞中p56 lck 是无活性的,其分子的505位点氨基酸被磷酸化。p56 lck 激酶活性的升高是与Tyr505去磷酸化相一致。CD45可使有活性激酶中主要自身磷酸化Tyr395发生去磷酸化,从而引起激酶活性的抑制。 2.MAP-2K的去磷酸化 T细胞活化中,CD45另一种可能催化的底物是丝氨酸特异的微管相关蛋白-2激酶(serine-specific microtubule-associated protein
Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions
organization. (A ) One box of 48 secretome protein master stock vials for storage at −20°C is shown. (B ) Samples from two master stock boxes are transferred to one 96‐well stock plate as shown. (C ) Samples from two 96‐well plates are transferred to one 384
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