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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Hamartin, Tsc1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Hamartin, Tsc1 ELISA KIT
Product Name:Mouse Hamartin, Tsc1 ELISA KIT
Packing:96T
Catalog No.:ELI-51213m
Gene Name:Mouse Tsc1
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Tsc1
Alternative Name:Mouse Hamartin, Tsc1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Hamartin, Tsc1 ELISA KIT allows for the in vitro quantitative determination of Mouse Tsc1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Hamartin, Tsc1 ELISA KIT has been pre-coated with an Mouse Hamartin, Tsc1 antibody specific to Mouse Tsc1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tsc1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tsc1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tsc1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验. The TSC1 gene contains 23 exons with 21 exons coding for a 8.6 kb transcript which is translated into the protein hamartin. The TSC2 gene product tuberin is encoded by an mRNA of 5.5 kb consisting of 41 exons.
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
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