Gap junction beta-5 protein, G
jb5 ELISA KIT/ Mouse Gap junction beta-5 protein, Gjb5 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-50635m
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Gap junction beta-5 protein, Gjb5
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Gap junction beta- 5 protein, Gjb5 ELISA KIT
Product Name:Mouse Gap junction beta- 5 protein, Gjb5 ELISA KIT
Packing:96T
Catalog No.:ELI-50635m
Gene Name:Mouse Gjb5
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Gjb5
Alternative Name:Mouse Gap junction beta-5 protein, Gjb5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Gap junction beta- 5 protein, Gjb5 ELISA KIT allows for the in vitro quantitative determination of Mouse Gjb5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Gap junction beta- 5 protein, Gjb5 ELISA KIT has been pre-coated with an Mouse Gap junction beta-5 protein, Gjb5 antibody specific to Mouse Gjb5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Gjb5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Gjb5 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Gjb5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Transgenic Mouse and Transgenic Rat Outline
in the mouse. There are several ways to achieve this. You may use a reporter gene such as a fluorescent protein that you can visualize directly. You may use a reporter gene that provides for enzymatic amplification of the signal such as beta
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
, axin, and the tumor suppressor, adenomatouspolyposis coli (APC) protein (3) . In the absence of Wnt signaling,GSK-3 targets b-catenin for ubiquitin-mediated proteosomaldegradation by phosphorylation of b-catenin, axin, and APC (3) . Phosphorylation
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