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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cytoplasmic tRNA 2-thiolation protein 1, Ctu1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cytoplasmic tRNA 2- thiolation protein 1, Ctu1 ELISA KIT
Product Name:Mouse Cytoplasmic tRNA 2- thiolation protein 1, Ctu1 ELISA KIT
Packing:96T
Catalog No.:ELI-50629m
Gene Name:Mouse Ctu1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Ctu1
Alternative Name:Mouse Cytoplasmic tRNA 2-thiolation protein 1, Ctu1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cytoplasmic tRNA 2- thiolation protein 1, Ctu1 ELISA KIT allows for the in vitro quantitative determination of Mouse Ctu1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cytoplasmic tRNA 2- thiolation protein 1, Ctu1 ELISA KIT has been pre-coated with an Mouse Cytoplasmic tRNA 2-thiolation protein 1, Ctu1 antibody specific to Mouse Ctu1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ctu1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ctu1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ctu1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Antibody Production: Activity-Dependent Neuroprotective Protein (ADNP) as an Example
in incomplete Freund’s adjuvant, the respective antisera were collected and assayed by enzyme-linked immunosorbent assay (ELISA) and purified by affinity chromatography on peptides conjugated to SulfoLink Coupling Gel (Pierce). Mouse brain proteins were prepared
Introduction Techniques routinely used in our lab for analysing the X-inactivation process in differentiating ES cells are described. We focus particularly on chromatin changes (histone modifications, protein association
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
into nuclearand cytoplasmic fractions using the NE-PER kit (as directed bymanufacturer). Take an aliquot of each sample to determine theconcentration of protein using Bradford solution. 5. When loading the gels, cytoplasmic and nuclear fractions areloaded
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