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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Transcription factor AP-2-epsilon, Tcfap2e
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Transcription factor AP- 2- epsilon, Tcfap2e ELISA KIT
Product Name:Mouse Transcription factor AP- 2- epsilon, Tcfap2e ELISA KIT
Packing:96T
Catalog No.:ELI-49574m
Gene Name:Mouse Tcfap2e
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Tcfap2e
Alternative Name:Mouse Transcription factor AP-2-epsilon, Tcfap2e
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Transcription factor AP- 2- epsilon, Tcfap2e ELISA KIT allows for the in vitro quantitative determination of Mouse Tcfap2e concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Transcription factor AP- 2- epsilon, Tcfap2e ELISA KIT has been pre-coated with an Mouse Transcription factor AP-2-epsilon, Tcfap2e antibody specific to Mouse Tcfap2e .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Tcfap2e and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Tcfap2e , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Tcfap2e in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues
for the parallel analysis of the activities of more than 200 transcription factor proteins, using an advanced oligonucleotide array-based transcription factor assay (OATFA) platform, to assay TF activities in mice. The system uses a PCR-based system to translate
the rod-specific transcription factor NR2E3 and the cone/rod homeobox protein CRX as examples, we show that NR2E3 and CRX are co-enriched on the promoter of active Rho and Rbp3 genes in rods, but are present to a much lesser degree on the promoters
as a hormonal enhancer in vivo ( 1 ). Examination of the MMTV promoter stably maintained in mouse mammary cell lines revealed that the MMTV long terminal repeat (LTR) organized into a phased array of six precisely positioned nucleosomes ( A-F
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