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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Beta-3 adrenergic receptor, Adrb3
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Beta- 3 adrenergic receptor, Adrb3 ELISA KIT
Product Name:Mouse Beta- 3 adrenergic receptor, Adrb3 ELISA KIT
Packing:96T
Catalog No.:ELI-49555m
Gene Name:Mouse Adrb3
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Adrb3
Alternative Name:Mouse Beta-3 adrenergic receptor, Adrb3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Beta- 3 adrenergic receptor, Adrb3 ELISA KIT allows for the in vitro quantitative determination of Mouse Adrb3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Beta- 3 adrenergic receptor, Adrb3 ELISA KIT has been pre-coated with an Mouse Beta-3 adrenergic receptor, Adrb3 antibody specific to Mouse Adrb3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Adrb3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Adrb3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Adrb3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
beta-actin B-actin GAPDH(Human, rat and mouse) GAPDH(人/小鼠/大鼠 通用) GAPDH GAPDH/ NM_002046 GAPDH(大鼠、小鼠、人) β-actin(大鼠、小鼠、人) beta-actin beta-actin PCR or RT-PCR EBV BamHⅠ-W EBV Qp EBV Wp EBV Cp EBV Fp EBV LMP1 EBV LMP2A EBV
Flow Cytometry Assay to Measure Internalization of GPCRs
engineered onto the N-terminus of the GPCR.The following protocol is optimized for internalization of FLAG-tagged beta 2-adrenergic receptor (B2AR) stably transfected into HEK293 cells.Protocol 1) Grow up receptors in 37degC incubator until confluent
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