Amelogenin X isoform, Amelx EL

Mouse Amelogenin X isoform, AMELX ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

AMELX,AI1E; AIH1; ALGN; AMG; AMGL; AMGX; amelogenesis imperfecta 1; amelogenin X chromosome, amelogenesis imperfecta 1; amelogenin amelogenesis imperfecta X-linked; amelogenin X isoform; amelogenin X-linked

Search name

Mouse AMELX ELISA KIT ,Mouse AI1E ELISA KIT ,Mouse AIH1 ELISA KIT ,Mouse ALGN ELISA KIT ,Mouse AMG ELISA KIT ,Mouse AMGL ELISA KIT ,Mouse AMGX ELISA KIT ,Mouse amelogenesis imperfecta 1 ELISA KIT ,Mouse amelogenin X chromosome ELISA KIT ,Mouse  amelogenesis imperfecta 1 ELISA KIT ,Mouse amelogenin amelogenesis imperfecta X-linked ELISA KIT ,Mouse amelogenin X isoform ELISA KIT ,Mouse amelogenin X-linked ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse amelogenin, x isoform,AMELX concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to AMELX. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AMELX and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AMELX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AMELX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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